Mechanism of Inhibition of Botrytis cinerea Spore Germination by Antimicrobial Compounds of Schisandra chinensis

Abstract

Botrytis is an efficient pathogen, which causes devastating diseases and serious crop losses in many plants. Traditionally, chemical control of Botrytis is still mainly dependent on the use of chemical insecticides, but chemical pesticide is prone to environmental pollution and drug resistance, so there is an urgent need to develop pollution-free control means. This article reports a methods for retrieving the antimicrobial compounds from Schisandra chinensis by Soxhlet extractor reflux, and using orthogonal experimental design with alcohol concentration, alcohol consumption, extraction time and extraction times as factors. Effects of antimicrobial compounds of Schisandrae chinensis inhibition Botrytis cinerea spore germination was tested by glass slide. The results showed that the antimicrobial compounds of Schisandra chinensis had a strong inhibitory effect on spore germination of Botrytis cinerea. The antimicrobial compounds obtained under the experimental conditions of 5 times the amount of ethanol (95%), 3 times of reflux extraction, each time two hours could best inhibit germination of Botrytis cinerea, with the inhibition rate up to 100%.